External factors
| External factors: |
Nutlin-3a |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Cell name: |
Fibroblast,GM08402,NHF-hTERT,IMR-90,MRC-5 |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
A high percentage of cells that had been treated with nutlin-3a were stained positive for the low pH β-gal,indicating cells had undergone senescence.All three cell strains showed a morphologically senescent phenotype after nutlin-3a treatment. Again, almost 100% of the cells showed positive staining. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Regulatory relationship
| Target gene:
|
P53//MIR-34A//MIR-34B//MIR-34//ING2 |
| R-EF-Target gene:
|
Activation//Upregulation//Upregulation//Upregulation//Downregulation |
| Official symbol(s): |
P53//MIR-34A//MIR-34B//MIR-34//ING2 |
| Target gene experiment: |
RT-PCR//qRT-PCR//Western blot |
| Target gene description: |
In NHF-hTERT cells, the total amount of p53 expression was increased by nutlin-3a. In contrast, there was no increase of p53 expression with nutlin-3a treatment in the p53 null cells.mir34a, mir34b, and mir34c levels were shown to be up-regulated to varying amounts in various human tumor cell lines that were wild type for p53 after treatment with nutlin-3a after 24 hours. Interestingly, ING2 expression in the cells treated with nutlin-3a was remarkably decreased compared with nontreated cells while ING2 expression was not changed in nutlin-3b–treated cells.ING2 mRNA transcripts were consistently decreased by nutlin-3a treatment in all cell lines using a real-time PCR method. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
471
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
472
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 751
Warning: Trying to access array offset on null in /data/html/Aging-ReG/public/browse_detail_2.php on line 754
Warning: Undefined variable $pathway_id in /data/html/Aging-ReG/public/browse_detail_2.php on line 793