External factors
| External factors: |
Methoxyamine |
| Category: |
Chemical compounds |
| Phenotype: |
Colorectal cancer |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
Examined senescence-activated β-gal activity following these treatments. Senescence-activated β-gal activity was examined on days 5 to 8 following ionizing radiation and was found to be positive in IUdR- and IUdR/methoxyamine–pretreated cells. Spot senescence-activated β-gal positive cells were also found in the control and methoxyamine-pretreated cells;During the course of the experiments, we noted that after ionizing radiation, IUdR- and IUdR/methoxyamine–pretreated cells exhibited senescence-like morphologic features . |
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Regulatory relationship
| Target gene:
|
P53//P21//RB |
| R-EF-Target gene:
|
Upregulation//Upregulation//Activation |
| Official symbol(s): |
P53//P21//RB |
| Target gene experiment: |
Western blot |
| Target gene description: |
Indeed, both accumulation of p53 and induction of p21, the major mediator of p53-dependent senescence, increase more significantly in IUdR/methoxyamine–pretreated cells at both early and later times following ionizing radiation. Furthermore, IUdR/methoxyamine–pretreated cells show reduced hyperphosphorylated Rb(S780) (inActivate form) but greater hypophosphorylated Rb (Activate form) compared with IUdR-pretreated cells . |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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