External factors
| External factors: |
Cisplatin |
| Category: |
Chemical compounds |
| Phenotype: |
Chronic kidney disease |
| Experiment: |
Cell morphological analysis//SA-β-gal activity assay//Flow cytometry//BrdU assay |
| Description: |
NRK cells became enlarged and flattened morphologically. Positive staining for SA-β-Gal was observed at 24 h and increased to 15% at 48 h and 22% at 72 h.Another marker of senescence, C12FDG was also positive in cisplatin-treated cells and most obvious in 20 μM group. Cell cycle analysis showed that 20 μM cisplatin resulted in a marked increase in the percentage of cells in S phase. EdU was detected in cisplatin treated renal tubular cells. There were fewer EdU-positive cells in cisplatin-treated renal tubular cells compared with control group. |
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Regulatory relationship
| Target gene:
|
P53//P21//SIRT1 |
| R-EF-Target gene:
|
Upregulation//Upregulation//Downregulation |
| Official symbol(s): |
P53//P21//SIRT1 |
| Target gene experiment: |
Western blot |
| Target gene description: |
The p53 and p21 proteins, which are senescence modulators, were upregulated at 48 h after cisplatin treatment .Assessment of SIRT1 levels showed that cisplatin treatment downregulated SIRT1 at the protein level in NRK cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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