External factors
| Experiment: |
SA-β-gal activity assay//Flow cytometry//PI staining//Annexin V binding assay |
| Description: |
This was termed senescence-associated betagalactosidase or SA-β-gal. Our study revealed strong activity of SA-β-gal in UVB-irradiated HDFs, which, as one of the biomarkers of senescence, indicated that cells were induced to a senescencelike state by UVB exposure. As shown by PI staining and the annexin V method, we found UVB irradiation caused cell cycle arrest, with most HDFs being arrested on G0/G1 phase, and it might play a key role in aging process by initiating cell aging. |
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Regulatory relationship
| Target gene:
|
P66SHC//FKHRL1 |
| R-EF-Target gene:
|
Upregulation//Upregulation |
| Official symbol(s): |
P66SHC//FOXO3 |
| Target gene experiment: |
Western blot//qRT-PCR |
| Target gene description: |
p66Shc and FKHRL1 were significantly up-regulated by UVB irradiation. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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