External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//BrdU assay//Cell morphological analysis//Flow cytometry |
| Description: |
Consistent with previous reports, normal cells (HDFs and IMR-90) G1 arrest with no change in BrdU-positive cell number after H2O2 treatment. At 5 days after H2O2 exposure, HDFs showed senescent-like morphology, including flattened, irregular shape and enlarged size, as well as senescence associated (SA)-β-galactosidase activity. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Target gene experiment: |
BrdU assay//Western blot//Flow cytometry |
| Target gene description: |
We further examined whether the PCNA protein levels were changed after treatment of HDFs and IMR-90 cells with sublethal concentrations of H2O2. PCNA levels were decreased at 30 min, reached lowest levels at 1 h, and returned to normal levels at 2 h after H2O2 exposure,consistent with both cell types. This result suggests that transient PCNA downregulation cause cell cycle arrest and inhibition of DNA synthesis in normal cells. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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