External factors
| External factors: |
Adriamycin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
Adr but not Etop induced cellular senescence . |
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Regulatory relationship
| Target gene:
|
PERK//P53//RKIP |
| R-EF-Target gene:
|
Downregulation//Upregulation//Upregulation |
| Official symbol(s): |
EIF2AK3//P53//RKIP |
| Target gene experiment: |
Western blot |
| Target gene description: |
As the A549 cell line is p16/p14 negative, these results suggested that Adr induced cellular senescence in a p16/Rb-independent manner. In contrast to reduction of p-ERK and increase of SA-β-Gal positive cells in response to Adrin p53-positive cell lines, p53-null cells (HCT116 p53 -/- ) did not show the reduction of p-ERK and senescence.However, reconstruction of p53 in HCT 116 p53-/-cells by transfection of wild-type p53 could restore the Adr-induced senescence. As we expected, Adr treatment induced the RKIP expression along with suppression of p-ERK . Previously, we showed that reduction of p-ERK showed the dependence with p53, and RKIP was induced by Adr. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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