External factors
| External factors: |
Ribociclib |
| Category: |
Chemical compounds |
| Phenotype: |
Adrenocortical carcinomas |
| Experimental category: |
HL |
| Cell name: |
SW 13 ,NCI-H295R |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
In SW-13 cells, 1 and 5 μM of both inhibitors induced cell cycle arrest, with a smaller proportion of cells in the S phase and an increased proportion of cells in the G1 and G2 phases, when compared with mock-treated cells. The cycle of NCI-H295R cells was also affected by drug treatments. 10 μM Palbociclib increased the proportion of cells in G2 phase, whereas 10 μM ribociclib increased the proportion of cells in S phase.In SW-13 cells treated with palbociclib or ribociclib, we observed a significant increase in the percentage of cells harboring β-galacto-sidase activity, an indicator of senescence. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
PHOSPHO-RB |
| Target gene experiment: |
Western blot |
| Target gene description: |
In SW-13 cells, the amount of both CDK4 and CDK6 proteins increased after treatment with palbociclib or ribociclib. Such treatments also significantly lowered the amounts of both Phospho-Rb and pRB. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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