External factors
| Phenotype: |
Diabetes kidney disease |
| Experiment: |
Immunostaining//SA-β-gal activity assay//SAHF//PI staining |
| Description: |
Double immunofluorecent staining demonstrated that p16 positive cells were colocalized in the AGE-positive cells.P16-siRNA considerably reduced the ratio of SA-β-gal and SAHF-positive RTECs as well as the proportion of RTECs in the G0/G1 phase in AGE-BSA treated group . |
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Regulatory relationship
| Regulatory pathway:
|
ATF4-p16 |
| Official symbol(s): |
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ATF4-CDKN2A
|
| Pathway experiment: |
Immunostainings//Western blot |
| Pathway description: |
Linear correlation analyses showed that nuclear ATF4 expression was positively correlated with AGE deposition.The expression of ATF4 and p16 was co-localized in the nucleus of the same SAHF positive cells . ATF4 siRNA resulted in a significant decrease in AGE-induced p16 expression and premature senescence of PTECs. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.