External factors
| External factors: |
Pterostilbene |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//Western blot |
| Description: |
Consistent with the mild inhibition of cell growth, treatment of A549 cells with relatively low concentrations of PT (5 and 10 μM) in the presence of 10% serum did not perturb cell cycle progression at 24 hr. However, treatment of A549 cells with the same concentration of PT in the absence of serum induced S-phase arrest .cell senescence was well induced by 72 hrs treated PT in HBECR cells than in HBECR/p53i cells, in a dose dependent manner in two different assays.A senescence inducer, p21 and p53 amount were increased in HBECR. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
ATM-ATR-CHK1-p53 |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
ATM-ATR-CHK1-TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
The ATM/ATR-CHK1/2 axis is activated upon treatment with low doses of PT, an effect that is indicative of replication stress. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Related pathway view