External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
Moreover, only in KB cells ST1968 produced a dose-dependent appearance of β-galactosidase-positive cells at 72 h following treatment. The emergence of a senescence phenotype likely reflected a persistent cell cycle arrest. Indeed, as previously reported [10], treatment of A2780 cells with ST1968 resulted in a moderate and time-dependent arrest in G2, but in a complete accumulation of KB cells in G2/M (around 90% at 72 h). |
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Regulatory relationship
| Regulatory pathway:
|
ATM-Chk2//ATR-Chk1 |
| R-EF-Pathway:
|
Activation//Activation |
| Official symbol(s): |
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ATM-CHEK2//ATR-CHEK1
|
| Pathway experiment: |
Western blot//TUNEL assay |
| Pathway description: |
Under these conditions the ATM inhibitor produced in A2780 cells an enhancement of the apoptosis induced by the camptothecin also supported by CPP32 cleavage. As expected the combination resulted in a substantial reduction of Chk2 phosphorylation at thr68.in KB cells the Chk1 inhibitor enhanced the ST1968-induced appearance of mitotic and apoptotic cells and almost completed eliminated the cells with senescence features. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.