External factors
| External factors: |
MLN8237 |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis//Cytokine array |
| Description: |
Indeed, after 5 days of treatment in vitro,we observed that the cellular size was greatly enlarged ,which is a characteristic associated with senescence.β-galactosidase activity was evaluated and found to be enhanced in drug-treated Hs294T cells and in other melanoma cell lines (SK-Mel-2, SK-Mel-5 and SK-Mel-28).The results demonstrated that IL-6, IL-7, IL-10, GM-CSF, IL-8, RANTES, GRO and GRO-a were upregulated in response to drug treatment. |
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Regulatory relationship
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| Regulatory pathway:
|
ATM-Chk2//NF-kB |
| R-EF-Pathway:
|
Activation//Activation |
| Official symbol(s): |
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ATM-CHEK2//
|
| Pathway experiment: |
SA-β-gal activity assay//Knockdown//Luciferase reporter assay |
| Pathway description: |
We knocked down either ATM or Chk2 and found that senescence was reduced >30% in knockdown cells,indicating that the ATM/Chk2 pathway mediates MLN8237-induced senescence.An NF-kB luciferase assay was also performed using Hs294T NF-kB reporter cells. NF-kB transcriptional activity was significantly increased after treatment with 1mM MLN8237 for 5 days . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Pathway view
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