External factors
| External factors: |
γ-irradiation |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
Chronic c-irradiation efficiently induced cellular senescence and 0.694 mGy/min was more potent than 0.347 mGy/min in this assay. This result was consistent with results obtained using the colony survival assay. |
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Regulatory relationship
| Regulatory pathway:
|
ATM-p53-p21 |
| Official symbol(s): |
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ATM-CDKN1A-TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
TP53 or p21 levels were knocked down in BJ1/hT fibroblasts. This treatment abolished the cell-proliferation inhibition induced by chronic c-irradiation .The ATM inhibitor KU55933 inhibited the phosphorylation of TP53 and checkpoint kinase 2 (CHEK2) (ATM kinase substrates) and reduced p21 induction in response to c-irradiation. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.