External factors
| Experiment: |
SA-β-gal activity assay//Cell viability assay//MTT assay |
| Description: |
SA-β-gal activity assay:U87-MG cells exposed to 250 or 500 μM CuSO4 presented an enlarged cellular volume and altered shape, resembling the typical senescent phenotype. Cell viability assay:With increasing concentrations of copper sulfate, the cell viability was accordingly decreased . MTT assay:the 250 or 500 μM copper sulfate group showed a dramatic decrease in cell proliferation when compared with the control group. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
Bmi-1 |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
BMI1
|
| Pathway experiment: |
Western blot//RT-PCR |
| Pathway description: |
CuSO4-treated cells (250 μM) showed increased protein levels of p21 and p16, but a decreased level of Bmi-1, when compared with the control.Cells exposed to 250 μM CuSO4 showed statistically significant overexpression of ApoJ,TGF-β1,p21 and p16 (4.0-, 1.7-, 3.2- and 2.4-fold, respectively) but a marked decrease in Bmi-1. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.