External factors
| External factors: |
AZD6738 |
| Category: |
Chemical compounds |
| Phenotype: |
Colorectal cancer |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Knockdown |
| Description: |
Cells were treated with a highly selective ATR inhibitor, AZD6738.Compared to control cells, both RNASEH2B KO and RNASEH2A KO cells exhibited increased β-galactosidase staining , indicating that ATRi treatment accelerates cellular senescence in RNASEH2-depleted cells. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
cGAS-STING |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
CGAS-STING1
|
| Pathway experiment: |
qRT-PCR |
| Pathway description: |
Expression levels of cGAS-STING target genes (e.g. IFN-β, ISG54 and CCL5) were higher in RNASEH2 deficient cells and could be further enhanced by ATRi treatment, which suggest the activation of cGAS-STING pathway may be another reason for increased cellular senescence caused by ATR inhibition. As it is reported that RNASEH2 deficiency could invoke cGAS-STING activation and because of the essential role of cGAS-STING pathway in cellular senescence30,31,56,57, we assume that the activation of cGAS-STING pathway may also contribute to senescence phenotype in our study. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.