External factors
| External factors: |
Dihydroartemisinin |
| Category: |
Chemical compounds |
| Phenotype: |
Hepatocellular carcinoma |
| Tissue type: |
Tumor tissue |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
To further explore the underlying mechanisms of DHA on HepG2.2.15 cells, cellular senescence was detected by senescence-associated-β-Galactosidase assay. And the associated protein expression was measured by western blot.Exposure of HepG2.2.15 cells to DHA for 48 hours increased the senescent cells compared with the control. |
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Regulatory relationship
| Regulatory pathway:
|
DDR//Akt-mTOR |
| Official symbol(s): |
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DDR1//AKT1-MTOR
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Compared with cells treated with vehicle, the p-ATM, p-ATR, γ-H2AX, P53 and P21 were elevated while the telomere shelterin TPP1 was significantly down-regulated in cells treated with DHA. These results demonstrated that DHA might induce HepG2.2.15 cellular senescence through DDR pathway.In the present study, some key molecules in AKT-mTOR signaling were also detected by western blot. The p-AKT, p-mTOR, p-p70S6K, p-4EBP1 were down-regulated and followed by autophagy associated protein LC3, p-AMPK, p62 increased obviously. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.