External factors
| External factors: |
Busulfan |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//BrdU assay |
| Description: |
The growth of WI38 cells was permanently arrested after 24 h treatment with BU,while control untreated cells proliferated exponentially.This suggestion is supported by the observation that cells briefly treated with BU exhibited a significant reduction in BrdU incorporation (BU, 3.6 ± 1.5% vs Ctrl, 45.1 ± 4.7%, P< 0.0001) and a drastic increase in SA-β-gal staining (BU, 75.4 ± 6.6% vs Ctrl, 9.7 ± 4.9%, P< 0.0001) as compared with control untreated cells at 11 days after the initial treatment . |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
ERK-p38 MAPK |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
MAPK1-MAPK14-MAPK
|
| Pathway experiment: |
Western blot//SA-β-gal activity assay//BrdU assay |
| Pathway description: |
BU treatment incited a moderate p53 activation, but strong Erk,p38, and JNK phosphorylation, in a time-dependent manner.It was found that inhibition of Erk or p38 abrogated BU-induced increase in SA-β-gal staining and reduction in BrdU incorporation and cell proliferation in WI38 cells as compared with the cells treated with BU alone . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.