External factors
| External factors: |
Estrogen |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
The role of estrogen on anti-senescence was verified by the decreased SA-β-gal positive cells and alleviated expression of senescence markers. |
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Regulatory relationship
| Regulatory pathway:
|
ERβ-SATB2 |
| Official symbol(s): |
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ESR2-SATB2
|
| Pathway experiment: |
Western blot//qRT-PCR//Knockdown//Colony formation assay |
| Pathway description: |
Higher expression levels of SATB2, Nanog, Sox2 and Oct4, were observed in BMSCs treated with estrogen relative to the control group.A reduction in colony formation was observed with both siRNA-ERβ and ICI182, 170 treatment. Consistent with reduced stemness, lower expression of Nanog, Sox2 and Oct4 was evident. In the siRNA-ERβ or ICI182, 170 groups, SATB2 expression decreased up to 0.5-fold, conversely, estrogen treatment induced SATB2 expression by up to 1-fold in BMSCs. Similar effects were also observed at the protein level. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.