External factors
| External factors: |
Thiostrepton |
| Category: |
Chemical compounds |
| Cell name: |
MCF-7,MDA-MB-436 |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
MCF-7 cells treated with thiostrepton exhibited SA-β-gal activity and their morphology changed, with the cells becoming enlarged and flattened.The SA-β-gal activity was determined to be increased in a dose-dependent manner, with the highest activity observed at 4 μM. MDA-MB-436 cells also exhibited senescence morphology, with positive SA-β-gal activity, after being exposed to thiostrepton. |
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Regulatory relationship
| Regulatory pathway:
|
FOXM1 |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
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FOXM1
|
| Pathway experiment: |
RT-PCR |
| Pathway description: |
After 24 h of thiostrepton exposure, MCF-7 cells exhibited downregulation of FOXM1. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.