External factors
| External factors: |
Irradiation |
| Phenotype: |
Dry-mouth syndrome |
| Tissue type: |
Submandibular Salivary Gland |
| Experiment: |
Cell morphological analysis//SA-β-gal activity assay//RT-PCR |
| Description: |
Histological analysis performed over 12 weeks post-irradiation revealed a gradual increase in the appearance of large cells with notably enlarged, hyperchromatic nuclei, morphologically reminiscent of cells undergoing senescence (28).Salivary glands collected 8 weeks post-irradiation showed substantially increased SA-βgal activity located largely in ductal cells and also in some acini.Real-Time PCR analysis of salivary glands 2 and 8 weeks post-irradiation revealed strongly increased expression of the senescence-associated markers (31), p21, p19ARF, DcR2, and also the SASP genes, IL-6 and PAI-1, up to 21-fold. |
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Regulatory relationship
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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IL6
|
| Pathway experiment: |
ELISA |
| Pathway description: |
Analysis of serum IL-6 protein levels by ELISA revealed strong elevation in IL-6 expression that appeared roughly 6 hours post-irradiation and lasted less than one day . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.