External factors
| External factors: |
27-hydroxycholesterol |
| Category: |
Chemical compounds |
| Phenotype: |
Neurodegenerative disease |
| Experiment: |
SA-β-gal activity assay |
| Description: |
In the present study, we demonstrated that 27HC induced apparent cellular senescence in nerve cells. Senescence-associated β-galactosidase(SA-β-Gal) assay revealed that 27HC induced senescence in both BV2 cellsand PC12 cells. |
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Regulatory relationship
| Regulatory pathway:
|
IL-6-STAT3 |
| Official symbol(s): |
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IL6-STAT3
|
| Pathway experiment: |
DCFH assay//Western blot//ELISA |
| Pathway description: |
Furthermore, we demonstrated that 27HC promoted the accumulation of cellular reActivate oxygen species (ROS) innervecells and subsequently activation of IL-6/STAT3 signaling pathway. Notably, treatment with the ROS scavenger N-acetylcysteine (NAC) markedly blocked 27HC-induced ROS production and activation of IL-6/STAT3. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.