External factors
| External factors: |
Probucol |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//MTT assay |
| Description: |
SA-β-gal activity assay//MTT assay:SA- -gal staining was decreased in probucol + tBHP stimulated cells compared with the tBHPstimulated cells (45.32% ± 4.13% and 81.03% ± 5.86%, respectively; p < 0.01). Cell cycle analysis indicated that the cell percentage at the G0?G1, S, and G2?M phases was normalized. Optical microscopic observation showed that cell ageing changes were not apparent. |
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Regulatory relationship
| Regulatory pathway:
|
JAK2?STAT |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
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JAK2-SOAT1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Western blot： The protein expression of STAT1, STAT3, pSTAT1, and pSTAT3 were also increased in ageing cells compared with those in control cells. When probucol and AG490 were used, their expression decreased. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.