External factors
| External factors: |
Dehydroepiandrosterone |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
When we pre-treated with 50 nmol/L of DHEA for 1h prior to LA treatment,the number of SA-β-Gal-positive cells significantly decreased compared to the cells treated with LA alone.However,pre-treatment with DHEA substantially increased the protein level of ppRB(an indicator of senescence). |
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Regulatory relationship
| Official symbol(s): |
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MAPK8
|
| Pathway experiment: |
SA-β-gal activity assay//Western blot |
| Pathway description: |
After HAECs were pre-treated with the JNK inhibitor, the inhibitory effect disappeared. Similarly, the increased expression of ppRB by DHEA treatment was also abolished after JNK inhibition. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.