External factors
| External factors: |
C2-ceramide |
| Category: |
Chemical compounds |
| Experiment: |
Cell cycle analysis//SA-β-gal activity assay |
| Description: |
C2-ceramide accelerated PTX-induced G2-phase arrest of H1299 cells,as indicated by the portion of G2 population increased from 27.9% (PTX-treated) to 35.4% (20CT, PTX/C2-ceramide-treated);The percentage of senescent cells was significantly increased when PTX and C2-ceramide were co-administered to the cells,indicating C2-ceramide helped sensitize PTX-induced premature senescence both in a dose-dependent manner and time-dependent manner. |
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Regulatory relationship
| Official symbol(s): |
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MAPK
|
| Pathway experiment: |
Western blot |
| Pathway description: |
To examine whether C2-ceramide sensitized cells to premature senescence through MAPK signaling pathways, protein levels of major stress-MAPKs (JNK and p38) and anti-apoptotic protein Bcl-2 were analyzed by western blotting. C2-ceramide treatment induced activation of p38 in a dose-responsive manner; Additionally, the activiation of JNKwas minor decreased with C2-ceramide treatment alone. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.