External factors
| External factors: |
Avenanthramide A |
| Category: |
Chemical compounds |
| Phenotype: |
Colorectal cancer |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
The mice in AOM/DSS group showed a high tumor burden in colon tissues, while AVN A treatment obviously suppressed AOM/DSS induced tumors. The proportion of cells with senescent cell morphology and SA-β-gal staining were increased in dose and time-dependent manner after exposed to indicated concentration of AVN A for 3, 5 or 7 days. The protein level of p21 increased in a dose-dependent manner when treated with AVN A for 3 days. |
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Regulatory relationship
| Regulatory pathway:
|
miR-129-3p-Pirh2-p53 |
| Pathway experiment: |
Western blot//qRT-PCR |
| Pathway description: |
AVN A treatment significantly elevated miR-129-3p expression in a dose-dependent manner. Overexpression of miR-129-3p dramatically suppressed mRNA level of Pirh2 in CRC cells, which was in agreement with our observations that AVN A treatment dramatically decreased the Pirh2 mRNA level .Moreover, western blot analysis also confirmed that the expression of Pirh2 was downregulated in miR-129-3p overexpressing cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.