External factors
| External factors: |
Terrein |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//MTT assay//Flow cytometry |
| Description: |
The biological marker of senescent status was determined by in situ staining and assay of SA β-gal activity. SIPS-HDF cells displayed a markedly high level of SA β-gal activity, while terrein significantly reduced SA β-gal activity.To investigate the effects of terrein on cell survival, aged HDF cells (PD61) were treated with terrein and subjected to oxidative stress by exposure to 150-μM H2O2. Cell survival was determined at 24 and 48 h. Terrein has not shown cellular toxicity without oxidative stress. Under the oxidative stress, aged HDF cells significantly decreased cell viability at more than 20%, while terrein treatment was associated with significantly increased cell viability in time- and concentration-dependent manners. |
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Regulatory relationship
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| Regulatory pathway:
|
Nrf2-ERK1/2-HO-1 |
| Pathway experiment: |
Western blot |
| Pathway description: |
In this study, Terrein decreased age-related inflammatory molecules and p-ERK1/2 signalling at the indicated time and concentration, as in young cells. Percentage bar graph shows densitometric result of western blot.To further evaluate the down-stream molecules of p-ERK1/2 signalling affected by terrein in the ageing process, the translocation of Nrf2 and NF-κB from the cytosol to the nucleus was examined, and HO-1 expression was determined as a means of gauging antioxidant status. Terrein augmented Nrf2 translocation into the nucleus in both aged-HDF cells and SIPS-HDF cells at 11th days, like PD98059.NF-κB translocation was inhibited by terrein in both aged and SIPS-HDF cells. Percentage bar graph shows densitometric result of western blot. HO-1 was overexpressed by terreintreated as well as PD98059-treated aged and SIPS-HDF cells. Percentage bar graph shows densitometric result of western blot . |
Aging network
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Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Pathway view
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