External factors
| External factors: |
Cholera toxin |
| Description: |
Eventually, exposure to CT-containing medium for up to 12 weeks also resulted in senescence of the lightly pigmented NHM-C melanocytes. |
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Regulatory relationship
| Regulatory pathway:
|
p16-CDK4-pRb |
| Official symbol(s): |
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CDKN2A-RB1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
pRb in NHM-C melanocytes was present in both hypo-and hyperphosphorylated forms through 3 weeks, whereas it was predominantly in the hypophosphorylated form at 6 weeks .In contrast, pRb in NHM-B melanocytes was mostly in the nonphosphorylated form by 3 weeks of CT treatment.At 6 weeks, CDK4-GST-Rb kinase activity was notably reduced in NHM-B as compared to NHM-C cells .Over a 6-week period, levels of CDK4 bound to p16 decreased dramatically in NHM-C melanocytes, whereas it remained almost unchanged in NHM-B cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.