External factors
| External factors: |
Doxorubicin |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
Flow cytometry//SA-β-gal activity assay |
| Description: |
Drug effect on the cell cycle was determined by flow cytometry. The population of cells in the S-phase decreased, whereas those in the G2-M increased and reached a peak at 10-7M doxorubicin.This was associated with an increased activity of the senescence-associated β galactosidase (SA-β-Gal), confirming that the cells were in senescence. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
CDKN1A
|
| Pathway experiment: |
Western blot//RT-PCR |
| Pathway description: |
Expression of p21/WAF1 was maximal at 10-7M doxorubicin followed by a sharp decrease starting at 5X10-7M.However, examination of p21/WAF1 expression by RT-PCR indicated that although this may be the case at intermediate drug concentrations (such as 5X10-7M doxorubicin), high drug concentrations seemed to exert an inhibitory effect p21/WAF1expression at the message level. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.