External factors
| External factors: |
Nutlin-3a |
| Category: |
Chemical compounds |
| Experiment: |
Clonogenic assays//SA-β-gal activity assay//BrdU Assay |
| Description: |
Moreover, clonogenic assays show that a combination of Nut and IR combined treatment leads to a synergistic inhibition of the clonogenic growth of H460 cells. Interestingly, the increased tumor cell killing by IR and Nut combined treatment correlates with the increased expression of senescence biomarkers(increased SA-β-gal staining and decreased BrdU incorporation) in irradiated lung cancer cells. |
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Regulatory relationship
| Official symbol(s): |
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TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Our data demonstrate that Nut treatment substantially increases IR-induced p53 activation and p21 expression in H460 cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.