External factors
| External factors: |
Nutlin-3a |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
SA-β-gal activity assay markedly increased in size, acquired flat morphology, and showed intense SA-β-Gal staining typical for senescent cells .In cubation of the lung cancer cell line H460 with 10 μmol/L nutlin-3a, the Activate enantiomer of nutlin-3, for 24 hours led to an effective cell cycle arrest primarily in G1 phase. |
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Regulatory relationship
| Official symbol(s): |
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TP53
|
| Pathway experiment: |
Knockdown//BrdU assay |
| Pathway description: |
Knockdown of p53 by specific siRNA that reduced p53 protein levels >85% compared with siRNA controls (data not shown) completely abolished nutlin-induced cell cycle arrest, confirming that it is driven by p53 activation. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.