External factors
| External factors: |
Wharton’s jelly extract |
| Experiment: |
SA-β-gal activity assay |
| Description: |
In the middle-phase (30 PD) of culturing, cells on uncoated plates began to change to a flattened and enlarged phenotype, while cells on the WJE-coated plates remained fibroblast-like.In the latephase (50 PD) of culturing, cells on uncoated plates exhibited a flat and hypertrophic phenotype, while most cells on WJE-coated plates remained spindle-shaped.All three samples of UC-MSCs cultured on uncoated plates showed a significant increase in the percentage of SA-β-gal-positive staining with passaging, while the proportion of senescent UC-MSCs cultured on WJE-coated plates increased slowly.Furthermore, for BMMSCs plated on WJE-coated plates, SA-b-gal positive cells were effectively reduced until 30 PD,then positive cells gradually increased (P,0.01, n = 3). |
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Regulatory relationship
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| Regulatory pathway:
|
p53//p16-pRb |
| R-EF-Pathway:
|
Downregulation//Downregulation |
| Official symbol(s): |
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TP53//
|
| Pathway experiment: |
Western blot |
| Pathway description: |
The expression of p53, p16INK4a, and pRb in UC-MSCs cultured on WJE-coated plates was decreased at 30 PD, 50 PD compared to cells cultured on uncoated plates.We concluded that the WJE-coated surface provides an ideal environment that efficiently suppresses p53 and p16INK4a/pRb expression in UC-MSCs to delay replicative senescence of MSCs. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Pathway view
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