External factors
| External factors: |
Simvastatin |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//PI staining//Flow cytometry |
| Description: |
Our results show that simvastatin-treated WM9 cells developed some aspects of cellular senescence, including increased senescence-associated β-galactosidase activity and a G1/S cell cycle arrest. |
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Regulatory relationship
| Regulatory pathway:
|
p53//p21 |
| R-EF-Pathway:
|
Upregulation//Upregulation |
| Official symbol(s): |
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TP53//CDKN1A
|
| Pathway experiment: |
Western blot//qRT-PCR |
| Pathway description: |
The p53 and p21 mRNA levels were significantly increased after treatment of WM9 cells with simvastatin at the concentrations of 0.25 and 1 μmol/L .We observed an increased expression of phospho-p53 and p21 protein levels in WM9 cells treated with simvastatin , which are in agreement with RT-qPCR data . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.