External factors
| Experiment: |
SA-β-gal activity assay |
| Description: |
After stained with SA-β-Gal, Cr(VI) treatment group showed large amount of positive stained cells with blue color indicating the occurrence of premature senescence . |
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Regulatory relationship
| Regulatory pathway:
|
p53-p21 |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot//qRT-PCR |
| Pathway description: |
The expression of p53 was about 9-fold higher after Cr(VI) treatment compared with control, indicating the activation of p53 in the senescent cells. Western blotting for senescence pathway analysis revealed that p53-p21WAF1/CIP1 pathway, but not Rb-p16INK4a pathway was involved in Cr(VI)-induced premature senescence. p53 and p21WAF1/CIP1 was up-regulated after Cr(VI) exposure. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.