External factors
| External factors: |
Ionizing radiation |
| Cell name: |
NSCLC,H460,A549 |
| Experiment: |
Western blot//SA-β-gal activity assay//BrdU Assay |
| Description: |
The results reveal a substantial increase in SA-β-gal positive senescent cells in irradiated lung cancer cells.Similar results were also observed in A549 cells. Moreover, high levels of p16 expression, another important biomarker of senescence [25], were detected in irradiated H460 cells. In addition, BrdU incorporation assays show that the senescent lung cancer cells are unable to synthesize DNA and incorporate BrdU. |
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Regulatory relationship
| Regulatory pathway:
|
p53-p21 |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Our findings here demonstrate that IR activates the p53–p21 pathway in a dose-dependent manner as evidenced by increased expression of phosphorylated p53 and p21 in irradiated H460 cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.