External factors
| External factors: |
LMWP-SOD1 |
| Experiment: |
SA-β-gal activity assay//Flow cytometry//MTT assay |
| Description: |
Control DPSCs (+H2 O2 ) and SOD1-treated DPSCs (+H2 O2 ) were 28.5% and 26.8% positive, respectively, for SA-β-gal staining ; however, LMWP-SOD1-treated DPSCs (+H2 O2 ) were 12.3% positive for SA-β-gal staining. LMWP-SOD1 slightly restored cell viability which had been inhibited by H2 O2. Similarly, for the control DPSCs (+H2 O2 ) and the SOD1-treated DPSCs (+H2 O2 ), the number of cells in G1 phase was increased, but the number of LMWP-SOD1-treated DPSCs (+H2 O2 ) in G1 phase slightly and significantly decreased compared with control DPSCs (+H2 O2 ) and SOD1-treated DPSCs . |
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Regulatory relationship
| Regulatory pathway:
|
p53-p21 |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot//RT-PCR |
| Pathway description: |
The control DPSCs (+H2 O2 ) and SOD1-treated DPSCs (+H2 O2 ) had no influence on mRNA expression, but LMWP-SOD1- treated DPSCs (+H2 O2 ) significantly abolished the H2 Oinduced increase in p53 and p21Cip1/WAF1 mRNA expression levels to the basal state. The p53 and p21Cip1/WAF1 protein levels were also quantified by Western blot analysis. As with the mRNA levels, treatment with LMWP-SOD1 partially abolished H2 O2 -induced p53 and p21Cip1/WAF1 protein expression in human DPSCs. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.