External factors
| External factors: |
5AZA-dC |
| Aging characteristic: |
Others |
| Category: |
Chemical compounds |
| Experiment: |
Western blot//Cell morphological analysis//SA-β-gal activity assay//Cell counting |
| Description: |
5AZA-dC-treated cells showed the expression of p16INK4A, enlarged cell size, growth arrest, and senescence associated β-gal staining. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
p53-p21 |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
TP53-CDKN1A
|
| Pathway experiment: |
Western blot |
| Pathway description: |
5AZA-dC-treated cells indeed showed upregulation of p53 and its downstream effector, p21WAF1, in addition to p16INK4a, and exhibited nuclear translocation of the p53 protein. Whereas only 10% 20% of control cells showed nuclear p53, 90% of the 5AZA-dC-treated cells showed strong p53 staining in the nucleus. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.