External factors
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay |
| Description: |
HDFs were pretreated with C. asiatica extracts or DMSO (control) for 4 h. After pretreatment, premature senescence was induced by 2-h treatment with 200 lm H2O2 and then the cells were transferred to DMEM media containing C. asiatica extracts and cultured for a further 48 h. The percentage of senescent cells increased significantly following treatment with 200 lm H2O2. |
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Regulatory relationship
| Regulatory pathway:
|
p53-p21 |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot |
| Pathway description: |
The results of Western blotting showed that H2O2 induced a robust increase in the expression of p53, p21 and pRb and that pretreatment with C. asiatica extracts inhibited the accumulation of these proteins . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.