External factors
| External factors: |
Bufalin |
| Category: |
Chemical compounds |
| Phenotype: |
Prostate cancer |
| Experimental category: |
HL |
| Experiment: |
Flow cytometry//SA-β-gal activity assay//Cell morphological analysis |
| Description: |
Cell cycle distribution analyses showed increased sub G0/1 apoptotic fraction at bufalin exposure of 20 nM.We examined cellular senescence phenotype in bufalin-exposed LNCaP (PTEN-negative, high AKT activity) cells by evaluating SA-β-gal activity as a cytochemical marker and observed increased proportion of cells with blue staining and flattened morphology. |
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Regulatory relationship
| Regulatory pathway:
|
p53-p21 |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Bufalin treatment for 48 h led to an increased total P53 protein abundance at 10-20 nM as well as that of P21CIP1 in a concentration-dependent manner. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.