External factors
| External factors: |
3-deazaneplanocin A |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis |
| Description: |
Senescence-like characteristics of HepG2 cells upon 5.0?μmol/L DZNep. (a) On day 3, the cells appeared normal in morphology; on day 6, the cells displayed senescence-like morphologies and positive SA-β-gal staining in green (observed at magnification of 400×). |
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Regulatory relationship
| Regulatory pathway:
|
p53-p21 |
| Official symbol(s): |
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TP53-CDKN1A
|
| Pathway experiment: |
Western blot |
| Pathway description: |
p53 activation and DDR caused by 5.0?μmol/L DZNep. Expression of p53 protein elevated after 3-day treatment.(b) Doxorubicin strongly induced p53 stabilization and nuclearization after 6?h, while DZNep causes p53 accumulation gradually until 72?h.mRNA expression of checkpoint inhibitors (p16, p21) upon DZNep treatment for 8 and 72?h. (c) Protein expression of checkpoint inhibitors (p16, p21) increased upon DZNep treatment on day 3. Quantification of the protein blot was performed by Image-Pro Plus (v6.0). transient activation of p53 in G2/M phase is sufficient to induce senescence together with p21 induction. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.