External factors
| External factors: |
Curcumin |
| Category: |
Chemical compounds |
| Experiment: |
Flow cytometry//SA-β-gal activity assay |
| Description: |
Cell cycle arrest in the G1 and G2 phase was observed already 24 h after treatment and lasted for several days. The activity of senescence associated-β galacto -sidase increased already on day 3 and after 7 days almost all cells were SA-β-gal positive. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
p53-p21//Rb |
| R-EF-Pathway:
|
Activation//Downregulation |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
TP53-CDKN1A//RB1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
We observed quite rapid (several hours after curcumin treatment) activation of the p53/p21 signaling pathway and a decrease in Rb. The latter protein almost completely disappeared after 7 days of treatment). |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.