External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Cell morphological analysis//RT-PCR |
| Description: |
Upon treatment with rapamycin,the percentage of AKT cells positive for SA-β-GAL was significantly reduced. Rapamycin treatment also dramatically reduced AKT-induced effects on cell size, and the SASP. |
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Regulatory relationship
| Regulatory pathway:
|
PI3K-Akt |
| Official symbol(s): |
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PIK3CB-AKT
|
| Pathway experiment: |
SA-β-gal activity assay//Cell morphological analysis//RT-PCR |
| Pathway description: |
To determine the contribution of enhanced mTORC1 signalling to AKT-induced senescence, BJ-T cells transduced with myr-AKT or control pBABE were treated with the mTORC1-selective inhibitor, rapamycin and markers of senescence analysed. Upon treatment with rapamycin,the percentage of AKT cells positive for SAbGAL was significantly reduced . Rapamycin treatment also dramatically reduced AKT-induced effects on cell size , and the SASP ,indicating that mTORC1 activity is critical for PI3K/AKT-driven senescence. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.