External factors
| External factors: |
Leptin/NAP-2 |
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
When cultured with UC‐MSCs, both leptin and NAP‐2 increased the percentage of SA β‐gal–positive cells and up‐regulated expression of p53 and p21 in a dose‐dependent manner. Strikingly, concomitant stimulation of UC‐MSCs with leptin and NAP‐2 further increased the frequency of SA β‐gal–positive cells (38.2?±?1.4% versus 24.3?±?0.6% with leptin stimulation alone and 26.0?±?2.0% with NAP‐2 stimulation alone) and increased expression of p53 and p21 compared with stimulation of cells with leptin or NAP‐2 alone, which suggests that these 2 factors act synergistically to affect MSC senescence. |
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Regulatory relationship
| Regulatory pathway:
|
PI3K-Akt |
| Official symbol(s): |
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PIK3CB-AKT
|
| Pathway experiment: |
SA-β-gal activity assay//Western blot |
| Pathway description: |
We indeed demonstrated a substantial, dose‐dependent elevation of phospho‐Akt expression in UC‐MSCs treated with leptin or NAP‐2 . Consistent with this, the senescence induced by leptin and/or NAP‐2 was almost completely reversed after LY294002 treatment, which was demonstrated by a decreased number of SA β‐gal–positive cells and by diminished levels of p53 and p21. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.