External factors
| External factors: |
High glucose |
| Experiment: |
SA-β-gal activity assay//Flow cytometry |
| Description: |
We found that the number of SA-β-Gal-positive cells significantly increased in a time-dependent manner, indicating that ARPE-19 might be senescent when treated with 25 mM glucose for 48 h. High glucose also triggered the cell cycle arrest in ARPE-19, and the cells in G0/G1 phase gradually increased from 63.91% to 78.42% after incubation of ARPE-19 with 25 mM high glucose for 48 h. |
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Regulatory relationship
| Regulatory pathway:
|
PI3K-Akt-mTOR |
| Official symbol(s): |
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PI3K-AKT-MTOR
|
| Pathway experiment: |
Western blot |
| Pathway description: |
We found the up-regulated expression of PI3K, p-AKT and p-mTOR in ARPE-19 treated with 25 mM glucose . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.