External factors
| External factors: |
Donepezil |
| Category: |
Chemical compounds |
| Phenotype: |
Alzheimer’s disease |
| Experiment: |
MTT assay//Western blot//qRT-PCR//SA-β-gal activity assay//Flow cytometry |
| Description: |
The rate of SA-?-gal-positive cells was significantly higher in the HG (30 mmol/L) group compared with the NG (5.6 mmol/L) group, and this increase was suppressed by treatment with donepezil in a concentrationdependent manner from 10 to 50 μM. Our results demonstrated that treatment with 30 mmol/L glucose arrested HUVECs in the G0/G1 phase as the proportion of cells in the G0/G1 phase was~69.9 % compared to 53.3 % in the NG (5.6 mmol/L) group. Donepezil (20 μM) pretreatment eliminated the effects of HG and reduced the proportion of cells in the G0/ G1 phase to 55.3 % . Next, we examined the impact of donepezil on cell viability. Treatment with HG (30 mmol/L) significantly suppressed endothelial cell viability, which was reversed by treatment with donepezil in a concentration dependent manner from 10 to 50 μM.HG treatment drastically increased the expression of PAI-1 and p21, which was markedly suppressed by donepezil treatment . This result was confirmed by Western blot analysis at protein levels. |
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Regulatory relationship
| Regulatory pathway:
|
SIRT1 |
| Official symbol(s): |
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SIRT1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Immunoblot analyses indicated that SIRT1 levels were decreased in response to treatment with HG (30 mmol/L), which was partially rescued by treatment with donepezil. SIRT1 deacetylase activity was reduced by treatment with HG. However, donepezil restored the deacetylase activity of SIRT1, indicating a direct effect on SIRT1-mediated pathways. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.