External factors
| External factors: |
Ergothioneine |
| Category: |
Chemical compounds |
| Phenotype: |
Type 2 diabetes mellitus |
| Cell name: |
Endothelial cell |
| Experiment: |
Cell morphological analysis |
| Description: |
The Egt treatment in the presence of hGluc (EgtthGluc) resulted in a morphological adjustment to the control cell-like small spindle shape. |
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Regulatory relationship
| Regulatory pathway:
|
SIRT1//SIRT6//NF-κB |
| R-EF-Pathway:
|
Upregulation//Upregulation//Downregulation |
| Official symbol(s): |
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SIRT1//SIRT6//NFKB1
|
| Pathway experiment: |
Confocal microscopy//Immunofluorescence//Western blot |
| Pathway description: |
Specifically, SIRT1 arbitrary fluorescence units (AFU) were significantly decreased by 48 h treatment with hGluc whereas EgtthGluc cells showed a SIRT1 fluorescence intensity significantly higher than that observed in hGluc cells.These effects were blocked by Egt and, more in details, SIRT6 expression in EgtthGluc cells was 78.373.1 AFU vs 49.273.1 AFU in hGluc cells, whereas NF-κB expression was 48.971.8 AFU vs. 12473.3 AFU in hGluc cells . Moreover, a reduced SIRT6 downregulation and a reduced NF-κB upregulation were observed during hGluc treatment in the presence of Egt (EgtthGluc). |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.