External factors
| External factors: |
Aspirin |
| Category: |
Chemical compounds |
| Phenotype: |
Colorectal cancer |
| Experiment: |
SA-β-gal activity assay//MTT assay |
| Description: |
Indeed, we found that aspirin induced cellular senescence, with maximum senescence-inducing effects observed at a concentration of 500 M in SW620 cells. We found that aspirin concentration-dependently reduced cell viability in both SW620 human metastatic colorectal carcinoma cells (p53 mutant-type) and HCT116 human primary colorectal carcinoma cells (p53 wild-type), and that it highly inhibited cell proliferation at concentrations greater than 1 mM . |
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Regulatory relationship
| Regulatory pathway:
|
SIRT1-AMPK |
| Official symbol(s): |
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SIRT1-PRKAA1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Aspirin treatment was found to dose- and time-dependently increase the protein levels of SIRT1, phospho-AMPK (p-AMPK T172), and p-ACC S79. Interestingly, the increase in SIRT1 protein expression was followed by AMPK activation. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.