External factors
| External factors: |
Genistein |
| Category: |
Chemical compounds |
| Tissue type: |
Umbilical cord |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
Pretreatment with genistein (1000?nM) inhibited the protein levels of P16 and P21, and decreased the activity of SA-β-gal. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
SIRT1-LKB1-AMPK |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
SIRT1-LKB1-AMPK
|
| Pathway experiment: |
SA-β-gal activity assay//Western blot//Knockdown |
| Pathway description: |
The effect of genistein on senescence and was restrained by SIRT1 siRNA, LKB1 siRNA, and AMPK siRNA.Ox-LDL ameliorated the phosphorylation of AMPK (Thr-172), and pretreatment with genistein elevated the phosphorylation of AMPK (Thr-172); meanwhile, the effect of genistein was abolished by SIRT1 siRNA or LKB1 siRNA. Compared with SIRT1 siRNA and LKB1 siRNA, control siRNA had no effect. These data ascertained that the effect of genistein could be associated with activating SIRT1/LKB1/AMPK pathway. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Related pathway view