External factors
| External factors: |
High glucose |
| Experiment: |
SA-β-gal activity assay |
| Description: |
Compared with NG, both HG and HN significantly increased the percentage of SA β-Gal staining cells by more than 2 fold. |
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Regulatory relationship
| Regulatory pathway:
|
SIRT1-p300-p53-p21 |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
CDKN1A-TP53
|
| Pathway experiment: |
SA-β-gal activity assay//Western blot |
| Pathway description: |
Compare to either NG or MN treated cells, exposure to HG resulted in a significant decrease in SIRT1 protein expression and its deacetylase activity .After transfection of pSIRT1, the protein expression and deacetylase activity of SIRT1 was significantly increased, while the protein expression of Ac-p53 and p21 were markedly decreased. In a similar manner, p300 siRNA transfection reduced the protein levels of p300, Ac-p53, and p21. It is interesting that SIRT1 over-expression suppressed the expression of p300 by nearly 50%, and p300 knock-down reciprocally increased the expression and activity of SIRT1. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Related pathway view