External factors
| External factors: |
Chitosan |
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay |
| Description: |
SA-β-gal activity assay:cellCompared to fibroblasts always on TCPS without culturing on chitosan (w/o treatment), cells after chitosan treatment for 3 days significantly decreased the percentage of SA β-gal-positive cells from about 20% to less than 10% cultured on chitosan for 5 DIV increased the percentage of SA β-gal-positive cells. |
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Regulatory relationship
| Regulatory pathway:
|
TGF-β |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
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TGFB1
|
| Pathway experiment: |
SA-β-gal activity assay//Western blot |
| Pathway description: |
SA-β-gal activity assay:The tendency of cell senescence increased with increasing the TGF-b concentration. Therefore, we assumed chitosan could suppress TGF-β signaling to delay the progress of fibroblast senescence.Western blot:compared to cells cultured on TCPS, only cells treated by chitosan showed significantly lower expression of TGF-b, Smad2/3 and pSmad2/3 . |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.