External factors
| External factors: |
Aβ1-42 oligomers |
| Category: |
Chemical compounds |
| Phenotype: |
Alzheimer's disease |
| Experiment: |
SA-β-gal activity assay// Western blot |
| Description: |
HBMECs exposed to Aβ1-42 oligomers appeared flattened and enlarged and showed increased senescence-associated β-galactosidase staining, exhibiting hallmark features of a senescent phenotype.Western blot analysis showed significantly increased p21 protein levels in the Aβ1-42 oligomer-treated HBMECs compared with control group. |
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Regulatory relationship
| Regulatory pathway:
|
VEGFR//p21-p53 |
| R-EF-Pathway:
|
Upregulation//Upregulation |
| Official symbol(s): |
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KDR//CDKN1A-TP53
|
| Pathway experiment: |
Western blot//qRT-PCR |
| Pathway description: |
Upon Aβ1-42 oligomer treatment, we observed a significant increase in VEGFR-1 protein levels, whereas protein levels of VEGFR-2 were not significantly altered.Western blot analysis showed significantly increased VEGFR-1 protein levels only in the Aβ1-42 oligomer-treated HBMECs but not in either Aβ1-42 monomers or Aβ1-42 fibrils-treated HBMECs .Western blotting analysis from chimeric receptor EGLT-transfected lysates confirmed the increased expression of the VEGFR-1 protein and increased p21 proteins levels and decreased expression of VEGFR-2 levels. qRT-PCR analysis showed increased mRNA levels of VEGFR-1, p21,and p53. There was a decrease in the p53 isoform Δ133p53 mRNA levels and no significant changes in p53b or VEGFR-2 mRNA levels.Western blot analysis showed increased levels of VEGFR-1 and p21 protein expression following Aβ1-42 oligomer treatment compared with control groups as previously shown. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.