External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Flow cytometry //Western blot |
| Description: |
After treatment with different doses of TCDD for 96 h, and the rate of SA-β-Gal positive astrocytes was significantly elevated in a dose-dependent manner.Flow cytometry showed that there were more astrocytes restricted in the G1 phase harvested from the TCDD-treated group 74.64 ± 2.9%(P <0.01) than the control group 63.49 ± 3.3%, and cell populations in the S phase decreased in the TCDD-treated group 23.39 ± 3.29% (P <0.01) compared with the control group 15.63 ± 2.05%. The protein levels of p16 and p21 were evidently increased in astrocytes treated with different doses of TCDD for 96 h in a dose-dependent manner. |
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Regulatory relationship
| Regulatory pathway:
|
WNT-β-catenin |
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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CTNNBIP1
|
| Pathway experiment: |
Western blot |
| Pathway description: |
β-catenin was significantly up-regulated by TCDD in a dose-dependent manner, and peaked at 100 nm TCDD exposure. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.